Resolving the Differences Between the 1.9 Å and 1.95 Å Crystal Structures of Photosystem II: A Single Proton Relocation Defines Two Tautomeric Forms of the Water‐Oxidizing Complex1. (27th April 2015)

• Record Type: Journal Article
• Authors: Petrie, Simon; Pace, Ron J.; Stranger, Rob
• Is Part Of: Angewandte Chemie. Volume 127:Number 24(2015)
• Page Start: 7226
• Page End: 7230
• Publication Date: 2015-04-27
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201502463
• Languages: English
• ISSNs: 0044-8249

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Great progress has been made in characterizing the water‐oxidizing complex (WOC) in photosystem II (PSII) with the publication of a 1.9 Å resolution X‐ray diffraction (XRD) and recently a 1.95 Å X‐ray free‐electron laser (XFEL) structure. However, these achievements are under threat because of perceived conflicts with other experimental data. For the earlier 1.9 Å structure, lack of agreement with extended X‐ray absorption fine structure (EXAFS) data led to the notion that the WOC suffered from X‐ray photoreduction. In the recent 1.95 Å structure, Mn photoreduction is not an issue, but poor agreement with computational models which adopt the 'high' oxidation state paradigm, has again resulted in criticism of the structure on the basis of contamination with lower S states of the WOC. Here we use DFT modeling to show that the distinct WOC geometries in the 1.9 and 1.95 Å structures can be straightforwardly accounted for when the Mn oxidation states are consistent with the 'low' oxidation state paradigm. Remarkably, our calculations show that the two structures are tautomers, related by a single proton relocation.</p> </abstract>