Brilliant cresyl blue staining negatively affects mitochondrial functions in porcine oocytes. Issue 3 (June 2015)
- Record Type:
- Journal Article
- Title:
- Brilliant cresyl blue staining negatively affects mitochondrial functions in porcine oocytes. Issue 3 (June 2015)
- Main Title:
- Brilliant cresyl blue staining negatively affects mitochondrial functions in porcine oocytes
- Authors:
- Santos, E.C.S.
Sato, D.
Lucia, T.
Iwata, H. - Abstract:
- <abstract abstract-type="normal"> <title>Summary</title> <p>The aim of the present study was to examine the effects of brilliant cresyl blue (BCB) staining on mitochondrial functions in porcine oocytes. Cumulus–oocyte complexes (COCs) collected from slaughterhouse-derived porcine ovaries were cultured with (13 μM) or without (0 μM, control) BCB for 60 min. Mitochondrial functions in oocytes were examined immediately after staining or after <italic>in vitro</italic> maturation. The BCB-stained oocytes produced reactive oxygen species (ROS) at higher levels than control oocytes immediately after staining (2.2-fold, <italic>P</italic> &lt; 0.001) and after maturation (1.7-fold, <italic>P &lt;</italic> 0.001). The adenosine triphosphate (ATP) content and mitochondrial membrane potential (MMP) in oocytes were similar for the two groups immediately after staining. However, ATP and relative MMP levels were significantly (<italic>P</italic> &lt; 0.05) lower in BCB-treated oocytes than in the control (2.18 versus 2.83 pM and 0.82 versus 1.0, respectively). There was no difference in mitochondrial DNA copy number between the two groups after maturation. The ATP content in early developmental stage embryos (3 days after parthenogenetic activation) was lower in the BCB-stained group than that in the control group but the difference was not significant. In conclusion, BCB staining of oocytes at the immature stage compromises mitochondrial functions throughout oocyte maturation, but<abstract abstract-type="normal"> <title>Summary</title> <p>The aim of the present study was to examine the effects of brilliant cresyl blue (BCB) staining on mitochondrial functions in porcine oocytes. Cumulus–oocyte complexes (COCs) collected from slaughterhouse-derived porcine ovaries were cultured with (13 μM) or without (0 μM, control) BCB for 60 min. Mitochondrial functions in oocytes were examined immediately after staining or after <italic>in vitro</italic> maturation. The BCB-stained oocytes produced reactive oxygen species (ROS) at higher levels than control oocytes immediately after staining (2.2-fold, <italic>P</italic> &lt; 0.001) and after maturation (1.7-fold, <italic>P &lt;</italic> 0.001). The adenosine triphosphate (ATP) content and mitochondrial membrane potential (MMP) in oocytes were similar for the two groups immediately after staining. However, ATP and relative MMP levels were significantly (<italic>P</italic> &lt; 0.05) lower in BCB-treated oocytes than in the control (2.18 versus 2.83 pM and 0.82 versus 1.0, respectively). There was no difference in mitochondrial DNA copy number between the two groups after maturation. The ATP content in early developmental stage embryos (3 days after parthenogenetic activation) was lower in the BCB-stained group than that in the control group but the difference was not significant. In conclusion, BCB staining of oocytes at the immature stage compromises mitochondrial functions throughout oocyte maturation, but function is restored during early embryo development.</p> </abstract> … (more)
- Is Part Of:
- Zygote. Volume 23:Issue 3(2015)
- Journal:
- Zygote
- Issue:
- Volume 23:Issue 3(2015)
- Issue Display:
- Volume 23, Issue 3 (2015)
- Year:
- 2015
- Volume:
- 23
- Issue:
- 3
- Issue Sort Value:
- 2015-0023-0003-0000
- Page Start:
- 352
- Page End:
- 359
- Publication Date:
- 2015-06
- Subjects:
- Developmental biology -- Periodicals
Embryology -- Periodicals
Zygotes -- Periodicals
Gametes -- Periodicals
571.864 - DOI:
- 10.1017/S0967199413000610 ↗
- Languages:
- English
- ISSNs:
- 0967-1994
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD Digital store
- Ingest File:
- 3488.xml