The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development. Issue 3 (June 2015)

• Record Type: Journal Article
• Authors: Kang, Sung-Sik; Ofuji, Sosuke; Imai, Kei; Huang, Weiping; Koyama, Keisuke; Yanagawa, Yojiro; Takahashi, Yoshiyuki; Nagano, Masashi
• Is Part Of: Zygote. Volume 23:Issue 3(2015)
• Page Start: 412
• Page End: 415
• Publication Date: 2015-06
• Subjects: Developmental biology -- Periodicals; Embryology -- Periodicals; Zygotes -- Periodicals; Gametes -- Periodicals; 571.864
• DOI: 10.1017/S096719941400001X
• Languages: English
• ISSNs: 0967-1994

Abstract

<abstract abstract-type="normal"> <title>Summary</title> <p>The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5–6 embryos/droplet) showed low blastocyst development compared with a control group (25–26 embryos/droplet). However, small and large numbers of embryos (5–6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25–30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.</p> </abstract>