Development and characterization of guar gum nanoparticles for oral immunization against tuberculosis. (May 2015)

• Record Type: Journal Article
• Authors: Kaur, Mandeep; Malik, Basant; Garg, Tarun; Rath, Goutam; Goyal, Amit K.
• Is Part Of: Drug delivery. Volume 22:Number 3(2015:Apr.)
• Page Start: 328
• Page End: 334
• Publication Date: 2015-05
• Subjects: Drug delivery systems -- Periodicals; Drug targeting -- Periodicals; 615.05
• DOI: 10.3109/10717544.2014.894594
• Languages: English
• ISSNs: 1071-7544

Abstract

<abstract> <title>Abstract</title> <p>The main aim of this study was to develop an effective carrier system containing Ag85A-loaded guar gum nanoparticles for oral vaccination against tuberculosis. Nanoparticles were prepared by Nanoprecipitation method. The developed particles with mean diameter 895.5 ± 14.73 nm and high antigen entrapment seem to be optimum for oral vaccine delivery. The acid protection assay, Peyer's patch uptake study and <italic>in-vitro</italic> antigen study confirmed that the developed formulations can protect the antigen from harsh gastric environment and can safely deliver the antigen to the intestinal region. <italic>In vivo</italic> studies data indicated that the developed nanocarriers can induce a strong mucosal as well as systemic immune response. Therefore, the experimental evidence suggests that guar-gum nanoparticle findings indicated that the guar gum nanoparticles can be utilized for safe and effective vaccine delivery via oral route.</p> </abstract>