Optimal production and characterization of alkaline protease from newly isolated halotolerant Jeotgalicoccus sp. Issue 2 (April 2015)

• Record Type: Journal Article
• Authors: Mokashe, Narendra; Chaudhari, Ambalal; Patil, Ulhas
• Is Part Of: Biocatalysis and agricultural biotechnology. Volume 4:Issue 2(2015)
• Page Start: 235
• Page End: 243
• Publication Date: 2015-04
• Subjects: Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2015.01.003
• Languages: English
• ISSNs: 1878-8181

Abstract

<abstract abstract-type="author" id="ab0005"> <title id="sect0005">Abstract</title> <sec> <p id="sp0040">A halotolerant strain UG was newly isolated from saline soil, produces an extracellular alkaline protease. The strain was identified as <italic>Jeotgalicoccus</italic> sp. on the basis of phylogenetic and phenotypic characteristics. The maximum protease yield (256.4±4.3 U/ml) was attained using optimized medium consists; peptone, K<sub>2</sub>HPO<sub>4</sub>, MgSO<sub>4</sub>·7H<sub>2</sub>O, CaCl<sub>2</sub> and NaCl; pH 10 at 37 °C within 40 h. The protease secretion was calcium ion dependent and enhanced by CaCl<sub>2</sub> supplementation in the production medium.</p> <p id="sp0045">A protease produced by <italic>Jeotgalicoccus</italic> sp. was most active at 48 °C and pH 10. The protease was purified by three step purification pattern which resulted in ~39 fold protease purification with a yield of 38%. The molecular weight of purified protease was estimated to be 51 kDa by SDS-PAGE. The protease was inhibited by EDTA, indicating that enzyme belongs to metalloprotease class. Protease showed better activity over a wide range of NaCl (0–1.5 M) and reasonable activity in presence of Ca<sup>2+</sup>, Mg<sup>2+</sup>, K<sup>+</sup>, Mn<sup>2+</sup>, Cd<sup>2+</sup> and Sn<sup>2+</sup>. Furthermore, the protease was active in the presence of H<sub>2</sub>O<sub>2</sub> and sodium perborate. Also, protease showed the substantial activity in the presence of rhamnolipid and cyclodextrin. The protease from <italic>Jeotgalicoccus</italic> sp. was suggested as a better bio-additive agent for detergent industry.</p> </sec> </abstract>