Biodegradation of pentachlorophenol by marine-derived fungus Trichoderma harzianum CBMAI 1677 isolated from ascidian Didemnun ligulum. Issue 2 (April 2015)

• Record Type: Journal Article
• Authors: Vacondio, Bruna; Birolli, Willian G.; Ferreira, Irlon M.; Seleghim, Mirna H.R.; Gonçalves, Sarah; Vasconcellos, Suzan P.; Porto, André L.M.
• Is Part Of: Biocatalysis and agricultural biotechnology. Volume 4:Issue 2(2015)
• Page Start: 266
• Page End: 275
• Publication Date: 2015-04
• Subjects: Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2015.03.005
• Languages: English
• ISSNs: 1878-8181

Abstract

<abstract abstract-type="author" id="ab0005"> <title id="sect0005">Abstract</title> <sec> <p id="sp0040">In this study, the marine-derived fungi <italic>Aspergillus sydowii</italic> DL6A, <italic>Apergillus versicolor</italic> DL5A, <italic>Cladosporium oxysporum</italic> DL5G, <italic>Fusarium proliferatum</italic> DL11A and <italic>Trichoderma harzianum</italic> CBMAI 1677 isolated from marine ascidian <italic>Didemnun ligulum</italic> were evaluated according to their growth in the presence of pentachlorophenol (PCP). The colonies were assessed in 10, 20, 30, 40 and 50 mg L<sup>−1</sup> of PCP in a solid culture medium (3% malt). The fungus <italic>T. harzianum</italic> CBMAI 1677 showed the best growth at 50 mg L<sup>−1</sup>, which suggests its potential for biodegradation, therefore, this strain was selected for quantitative experiments in 3% malt liquid medium (initial concentration of 20 mg L<sup>−1</sup> of PCP) using a validated method. After 7 d of incubation, PCP was not detected and an increasing concentration of pentachloroanisole (PCA) and 2, 3, 4, 6-tetrachloroanisole (2, 3, 4, 6-TeCA) was observed. In a second step, <italic>T. harzianum</italic> CBMAI 1677 was employed in the biodegradation of PCA and 2, 3, 4, 6-TeCA in a liquid medium. It was observed that both PCA and 2, 3, 4, 6-TeCA were also biodegraded. <italic>T. harzianum</italic> CBMAI 1677 is a potential strain for bioremediation studies since it was able of biodegrade not only PCP at 20 mg L<sup>−1</sup>, but also its main metabolites PCA and 2, 3, 4, 6-TeCA.</p> </sec> </abstract>