Process optimization of butanol production by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using palm oil mill effluent in acetone–butanol–ethanol fermentation. Issue 2 (April 2015)

• Record Type: Journal Article
• Authors: Al-Shorgani, Najeeb Kaid Nasser; Shukor, Hafiza; Abdeshahian, Peyman; Mohd Nazir, Mohd Yusuf; Kalil, Mohd Sahaid; Hamid, Aidil Abdul; Wan Yusoff, Wan Mohtar
• Is Part Of: Biocatalysis and agricultural biotechnology. Volume 4:Issue 2(2015)
• Page Start: 244
• Page End: 249
• Publication Date: 2015-04
• Subjects: Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2015.02.004
• Languages: English
• ISSNs: 1878-8181

Abstract

<abstract abstract-type="author" id="ab0005"> <title id="sect0005">Abstract</title> <sec> <p id="sp0040">Palm oil mill effluent (POME), an abundant and low-cost agro-industrial residue, was utilized for the production of butanol in the anaerobic process of acetone–butanol–ethanol (ABE) fermentation. The ABE process was performed by the cultivation of <italic>Clostridium saccharoperbutylacetonicum</italic> N1-4 (ATCC 13564) in raw POME medium without supplementation. The effect of various process parameters, including pH, incubation temperature, agitation and inoculums size on butanol production, was studied. Experimental results showed that the highest butanol levels could be produced when the initial pH of culture medium, incubation temperature, agitation speed and inoculums size were set at 5.8, 30 °C, 100 rpm and 15% (v/v), respectively. Results revealed that the existence of crude palm oil (CPO) in the POME composition had an adverse effect on butanol synthesis by Clostridial cells. The highest butanol and ABE concentrations of 0.9 g/L and 2.09 g/L were produced, respectively, when operating variables tested were set at their optimum levels under the absence of CPO.</p> </sec> </abstract>