A 24-chromosome FISH technique in preimplantation genetic diagnosis: validation of the method. (June 2015)

• Record Type: Journal Article
• Authors: Fernández, Silvia F.; Toro, Estefanía; Colomar, Ana; López-Teijón, Marisa; Velilla, Esther
• Is Part Of: Systems biology in reproductive medicine. Volume 61:Number 3(2015:Jun.)
• Page Start: 171
• Page End: 177
• Publication Date: 2015-06
• Subjects: Systems biology -- Periodicals; Andrology -- Periodicals; Generative organs, Male -- Diseases -- Periodicals; Biological systems -- Periodicals; Reproductive health -- Periodicals; Human reproduction -- Periodicals; 612.61
• DOI: 10.3109/19396368.2014.1002869
• Languages: English
• ISSNs: 1939-6368

Abstract

<abstract> <title>Abstract</title> <p>Embryo screening for aneuploidy (AS) is part of preimplantation genetic diagnostics (PGD) and is aimed at improving the efficiency of assisted reproduction. Currently, several technologies, including the well-established fluorescence <italic>in situ</italic> hybridization (FISH) technique, cover the screening of all chromosomes in a single cell. This study evaluates a novel 24-chromosome FISH technique protocol (FISH-24). A total of 337 embryos were analyzed using the traditional 9-chromosome FISH technique (FISH-9) while 251 embryos were evaluated using the new FISH-24 technique. Embryos deemed nontransferable on Day 3 were cultured <italic>in vitro</italic> to Day 5 of development, then fixed and reanalyzed according to the technique allocated to each treatment cycle (107 embryos analyzed by FISH-9 and 111 by FISH-24). The global error rate (discrepancy between Day 3 and Day 5 results for a single embryo) was 2.8% after FISH-9 and 3.6% after FISH-24, with a <italic>p</italic> value of 0.95. Thus, we have established and validated a 24-chromosome FISH-based single cell aneuploidy screening technique, showing that the error rate obtained for FISH-24 is independent of the number of chromosomes analyzed and equivalent to the error rate observed for FISH-9, as a useful tool for chromosome segregation studies and clinical use.</p> </abstract>