Isolation of New Delhi metallo‐β‐lactamase 1‐producing Vibrio cholerae non‐O1, non‐O139 strain carrying ctxA, st and hly genes in southern Vietnam. (May 2015)

• Record Type: Journal Article
• Authors: Diep, Tai The; Nguyen, Nhi Thi Ngoc; Nguyen, Thi Ngoc Cat; An, Huy Khac; Nguyen, Truong Quang; Nguyen, Vu Hoang; Nguyen, Thuong Van; Nguyen, Thu Ngoc Anh; Izumiya, Hidemasa; Ohnishi, Makoto; Yamashiro, Tetsu; Nguyen, Lan Thi Phuong
• Is Part Of: Microbiology and immunology. Volume 59:Number 5(2015:May)
• Page Start: 262
• Page End: 267
• Publication Date: 2015-05
• Subjects: Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12248
• Languages: English
• ISSNs: 0385-5600

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="mim12248-sec-0001" sec-type="section"> <p> <italic>Vibrio cholerae</italic> non‐O1, non‐O139 (VC_NAG) organisms are universally present in the aquatic environment and regarded as non‐pathogenic bacteria. However, considering that they do occasionally induce gastroenteritis, a study of their virulence and antibiotic resistance genes is important. The presence of enteropathogenic genes, including <italic>ctxA</italic>, VC_NAG‐specific heat‐stable toxin gene (<italic>st</italic>), hemolysin (<italic>hly</italic>), and zona occludens toxin (<italic>zot</italic>) was determined by PCR in 100 VC_NAG strains isolated in southern Vietnam in 2010–2013 from 94 environmental and six human origins. These 100 VC_NAG strains were also tested phenotypically and genotypically for the presence of the New Delhi metallo‐β‐lactamase (NDM‐1). Of the 100 VC_NAG strains tested, six were positive for <italic>ctxA;</italic> five from the environment and one of human origin. The <italic>st</italic> gene was detected in 17 isolates, 15 and two of which were of environmental and human origins, respectively. Gene <italic>hly</italic> was detected in 19 VC_NAG strains examined, two of which were isolated from humans and 17 from environments. The <italic>zot</italic> gene was not detected in any of the strains tested. Three VC_NAG strains of environmental origin were confirmed to produce NDM‐1 and the <italic>bla</italic><sub>NDM‐1</sub> gene was detected in those strains by PCR. Of note, one of the three NDM‐1‐producing VC_NAG strains was confirmed to carry <italic>ctxA</italic>, <italic>st</italic> and <italic>hly</italic> genes concurrently. This is the first report of isolation of NDM‐1‐producing VC_NAG strains in Vietnam.</p> </sec> </abstract>