Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis. (1st October 2014)

• Record Type: Journal Article
• Authors: Batista, D.I.S.; Perez, L.; Orfali, R.L.; Zaniboni, M.C.; Samorano, L.P.; Pereira, N.V.; Sotto, M.N.; Ishizaki, A.S.; Oliveira, L.M.S.; Sato, M.N.; Aoki, V.
• Is Part Of: Journal of the European Academy of Dermatology and Venereology. Volume 29:Number 6(2015:Jun.)
• Page Start: 1091
• Page End: 1095
• Publication Date: 2014-10-01
• Subjects: Dermatology -- Periodicals; Sexually transmitted diseases -- Periodicals; 616.5
• DOI: 10.1111/jdv.12753
• Languages: English
• ISSNs: 0926-9959

Abstract

<abstract abstract-type="main" id="jdv12753-abs-0001"> <title>Abstract</title> <sec id="jdv12753-sec-0001" sec-type="section"> <title>Background</title> <p>Atopic dermatitis (AD) in adults and profile of skin barrier proteins and inflammatory cytokines.</p> </sec> <sec id="jdv12753-sec-0002" sec-type="section"> <title>Objective</title> <p>Evaluation of the expression of skin barrier proteins such as filaggrin, claudins 1 and 4 and of circulating inflammatory cytokines (Th1/Th2/Th17) in adults with AD.</p> </sec> <sec id="jdv12753-sec-0003" sec-type="section"> <title>Methods</title> <p>Thirty‐three adult patients with AD diagnosed according to the Hanifin & Rajkacriteria, and 25 healthy controls were enrolled in the study. AD severity was measured by Eczema Area and Severity Index (EASI). Laboratory assays included immunohistochemistry analysis of skin barrier proteins, such as filaggrin, claudins 1 and 4 and interleukin‐17 (IL‐17) from skin samples and determination of circulating cytokine levels (IL‐2, 4, 5, 6, 10, 17A, TNF and IFN‐γ) by flow cytometry (Cytometric Bead Array).</p> </sec> <sec id="jdv12753-sec-0004" sec-type="section"> <title>Results</title> <p>We observed a reduced expression of filaggrin and claudin 1 in lesional skin of AD patients, when compared to controls. There was an inverse correlation of filaggrin expression and disease severity. In addition, IL‐17 expression was enhanced in AD patients. Similarly, higher levels of inflammatory cytokines (IL‐2, 5, 6, 10, 17A and IFN‐γ) were found in AD patients.</p> </sec> <sec id="jdv12753-sec-0005" sec-type="section"> <title>Conclusion</title> <p>Our data reinforce the role of an altered skin barrier in the pathogenesis of AD. Our results show not only reduced expression of filaggrin and claudin 1 in lesional atopic skin but also inverse correlation of filaggrin expression and disease severity. Moreover, elevation of <italic>in situ</italic> IL‐17 and of circulating interleukin levels in AD emphasize the systemic, inflammatory profile of this defective skin barrier dermatosis.</p> </sec> </abstract>