Microbial communities and nutrient dynamics in experimental microcosms are altered after the application of a high dose of Bti. Issue 3 (31st March 2015)

• Record Type: Journal Article
• Authors: Duguma, Dagne; Hall, Michael W.; Rugman‐Jones, Paul; Stouthamer, Richard; Neufeld, Josh D.; Walton, William E.; Wan, Shiqiang
• Is Part Of: Journal of applied ecology. Volume 52:Issue 3(2015)
• Page Start: 763
• Page End: 773
• Publication Date: 2015-03-31
• Subjects: Agriculture -- Periodicals; Biology, Economic -- Periodicals; Agricultural ecology -- Periodicals; Applied ecology -- Periodicals; 577
• DOI: 10.1111/1365-2664.12422
• Languages: English
• ISSNs: 0021-8901

Abstract

<abstract abstract-type="main" id="jpe12422-abs-0001"> <title>Summary</title> <p> <list id="jpe12422-list-0001" list-type="order"> <list-item> <p> <italic>Bacillus thuringiensis</italic> subsp. <italic>israelensis</italic> (<italic>Bti</italic>) is the most widely used biopesticide against mosquitoes and blackflies, with a history of high specificity and efficacy. High doses of <italic>Bti</italic> have been suggested for extended vector control in some environments; however, the effects of <italic>Bti</italic> application on the native microfauna in the environment are poorly understood.</p> </list-item> <list-item> <p>Two <italic>Bti</italic> (VectoBac G) treatments (high = 48·1 kg ha<sup>−1</sup>; low = 0·6 kg ha<sup>−1</sup>), in addition to an untreated control, were assigned to replicate 300 L microcosms.</p> </list-item> <list-item> <p> <italic>Culex</italic> abundance, phytoplankton biomass, sestonic particulates and nutrients were reduced significantly in the high <italic>Bti</italic> treatment. These changes affected other physicochemical variables in the water column during the 44‐day field study.</p> </list-item> <list-item> <p>Bacterial communities present in the water column were assessed by Illumina sequencing of 16S rRNA genes. The most abundant aquatic bacteria in microcosms subject to a low dose of <italic>Bti</italic> and untreated control, <italic>Cyanobacteria</italic>, <italic> Cytophagales</italic>, <italic> Cyclobacteriaceae</italic> (phylum <italic>Bacteroidetes</italic>) and <italic>Sphingomonas</italic> (class <italic>Alphaproteobacteria</italic>)<italic>, </italic> were suppressed in microcosms subject to a high dose of <italic>Bti</italic>. Bacteria in the high <italic>Bti</italic> treatment were dominated by <italic>Mucilaginibacter</italic>, <italic> Sediminibacterium</italic> (phylum <italic>Sphingobacteria</italic>) and <italic>Polaromonas</italic> (class <italic>Betaproteobacteria</italic>) and were more diverse than in the other treatments.</p> </list-item> <list-item> <p> <italic>Synthesis and applications</italic>. The biotic and abiotic changes resulting from a biopesticide application that significantly reduced mosquito abundance by more than 50% persisted longer than the period during which larval mosquito numbers were reduced. This warranted further investigation into the ecosystem‐level effects of <italic>Bti</italic> application rates used routinely for mosquito control. Application rates greater than the label rate for a <italic>Bti</italic> biopesticide can reduce mosquito abundance for an extended period of time and therefore lessen the operational cost of repeated application, but they could exceed manufacturer's recommendations and might violate country‐specific regulations governing biopesticide applications in natural habitats. Results of our study suggest that the widespread adoption of <italic>Bti</italic> application above the recommended label rate should be discouraged in habitats where algal abundance and its effect on primary production, microbial communities and nutrient cycling could affect the functioning of aquatic food webs.</p> </list-item> </list> </p> </abstract>