Abnormal Expression of miR-21 and miR-95 in Cancer Stem-Like Cells is Associated with Radioresistance of Lung Cancer. (28th May 2015)

• Record Type: Journal Article
• Authors: Zhang, Jinghui; Zhang, Chailan; Hu, Lin; He, Yin; Shi, Zeya; Tang, Siyuan; Chen, Yuxiang
• Is Part Of: Cancer investigation. Volume 33:Number 5(2015)
• Page Start: 165
• Page End: 171
• Publication Date: 2015-05-28
• Subjects: Cancer -- Periodicals; Oncology -- Periodicals; Medical Oncology -- Periodicals; Neoplasms -- Periodicals; 616.994
• DOI: 10.3109/07357907.2015.1019676
• Languages: English
• ISSNs: 0735-7907

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <p>This study demonstrated that miR-21 and miR-95 expression were significantly higher in the ALDH1⁺CD133⁺subpopulation than in the ALDH1^―CD133^― subpopulation of lung cancer cells. Combined delivery of anti-miR-21 and anti-miR-95 by calcium phosphate nanoparticles significantly inhibited tumor growth in a xenograft tumor model and sensitized radiotherapy. The anti-miRNAs significantly reduced miR-21 and miR-95 levels, increased PTEN, SNX1, and SGPP1 protein expression, but reduced Akt Ser⁴⁷³ and Thr³⁰⁸ phosphorylation. ALDH1⁺CD133⁺ subpopulation of NSCLC tumor cells confers radioresistance due to high expression of miR-21 and miR-95. Targeting inhibition of miR-21 and miR-95 can inhibit tumor growth through elevating PTEN, SNX1, and SGPP1 expression and inhibiting Akt phosphorylation.</p> </abstract>