Using [1‐13C]lactic acid for hyperpolarized 13C MR cardiac studies. Issue 6 (9th July 2014)

• Record Type: Journal Article
• Authors: Chen, Albert P.; Lau, Justin Y.C.; Alvares, Rohan D.A.; Cunningham, Charles H.
• Is Part Of: Magnetic resonance in medicine. Volume 73:Issue 6(2015:Jun.)
• Page Start: 2087
• Page End: 2093
• Publication Date: 2014-07-09
• Subjects: Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.25354
• Languages: English
• ISSNs: 0740-3194

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mrm25354-sec-1001" sec-type="section"> <title>Purpose</title> <p>Hyperpolarized [1‐<sup>13</sup>C]lactate in solution may be a clinically relevant and safe substrate for real time MR investigations of key metabolic pathways. The potential of using hyperpolarized [1‐<sup>13</sup>C]lactate for magnetic resonance studies of cardiac metabolism in vivo was explored.</p> </sec> <sec id="mrm25354-sec-2001" sec-type="section"> <title>Methods</title> <p>Neat [1‐<sup>13</sup>C]lactic acid was hyperpolarized using the dynamic nuclear polarization process. Cardiac MR spectroscopy experiments were performed in vivo using hyperpolarized [1‐<sup>13</sup>C]lactate and [1‐<sup>13</sup>C]pyruvate in solutions.</p> </sec> <sec id="mrm25354-sec-3001" sec-type="section"> <title>Results</title> <p>A high degree of polarization was achieved for [1‐<sup>13</sup>C]lactate in solution (16.7%). <sup>13</sup>C‐bicarbonate was observed in rat hearts in vivo after either hyperpolarized [1‐<sup>13</sup>C]lactate or hyperpolarized [1‐<sup>13</sup>C]pyruvate was infused, but lower <sup>13</sup>C‐bicarbonate to substrate ratio was observed with hyperpolarized [1‐<sup>13</sup>C]lactate infusions. The response of <sup>13</sup>C‐bicarbonate signal as a function of hyperpolarized [1‐<sup>13</sup>C]lactate doses was also investigated and a saturation of <sup>13</sup>C‐bicarbonate signal was observed at the highest dose of [1‐<sup>13</sup>C]lactate used (0.69 mmol/kg).</p> </sec> <sec id="mrm25354-sec-4001" sec-type="section"> <title>Conclusion</title> <p>This study demonstrated that the use of neat [1‐<sup>13</sup>C]lactic acid as the DNP sample is a potential alternative to [1‐<sup>13</sup>C]pyruvic acid for cardiac hyperpolarized <sup>13</sup>C MR studies. Hyperpolarized [1‐<sup>13</sup>C]lactate may enable noninvasive assessment of cardiac PDH flux in cardiac patients in the near future. Magn Reson Med, 2014. © 2014 Wiley Periodicals, Inc. Magn Reson Med 73:2087–2093, 2015. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>