Osteoblast‐specific expression of the fibrous dysplasia (FD)–causing mutation GsαR201C produces a high bone mass phenotype but does not reproduce FD in the mouse. (June 2015)

• Record Type: Journal Article
• Authors: Remoli, Cristina; Michienzi, Stefano; Sacchetti, Benedetto; Consiglio, Alberto Di; Cersosimo, Stefania; Spica, Emanuela; Robey, Pamela G; Holmbeck, Kenn; Cumano, Ana; Boyde, Alan; Davis, Graham; Saggio, Isabella; Riminucci, Mara; Bianco, Paolo
• Is Part Of: Journal of bone and mineral research. Volume 30:Number 6(2015:Jun.)
• Page Start: 1030
• Page End: 1043
• Publication Date: 2015-06
• Subjects: Bones -- Metabolism -- Periodicals; Mineral metabolism -- Periodicals; 612.392
• DOI: 10.1002/jbmr.2425
• Languages: English
• ISSNs: 0884-0431

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jbmr2425-sec-0001" sec-type="section"> <p>We recently reported the generation and initial characterization of the first direct model of human fibrous dysplasia (FD; OMIM #174800), obtained through the constitutive systemic expression of one of the disease‐causing mutations, <italic>Gsα<sup>R201C</sup></italic>, in the mouse. To define the specific pathogenetic role(s) of individual cell types within the stromal/osteogenic system in FD, we generated mice expressing <italic>Gsα<sup>R201C</sup></italic> selectively in mature osteoblasts using the <italic>2.3kb Col1a1</italic> promoter. We show here that this results in a striking high bone mass phenotype but not in a mimicry of human FD. The high bone mass phenotype involves specifically a deforming excess of cortical bone and prolonged and ectopic cortical bone remodeling. Expression of genes characteristic of late stages of bone cell differentiation/maturation is profoundly altered as a result of expression of <italic>Gsα<sup>R201C</sup></italic> in osteoblasts, and expression of the Wnt inhibitor <italic>Sost</italic> is reduced. Although high bone mass is, in fact, a feature of some types/stages of FD lesions in humans, it is marrow fibrosis, localized loss of adipocytes and hematopoietic tissue, osteomalacia, and osteolytic changes that together represent the characteristic pathological profile of FD, as well as the sources of specific morbidity. None of these features are reproduced in mice with osteoblast‐specific expression of <italic>Gsα<sup>R201C</sup></italic>. We further show that hematopoietic progenitor/stem cells, as well as more mature cell compartments, and adipocyte development are normal in these mice. These data demonstrate that effects of <italic>Gsα</italic> mutations underpinning FD‐defining tissue changes and morbidity do not reflect the effects of the mutations on osteoblasts proper. © 2015 American Society for Bone and Mineral Research. © 2014 American Society for Bone and Mineral Research</p> </sec> </abstract>