Amphipathic DNA Origami Nanoparticles to Scaffold and Deform Lipid Membrane Vesicles1. (16th April 2015)

• Record Type: Journal Article
• Authors: Czogalla, Aleksander; Kauert, Dominik J.; Franquelim, Henri G.; Uzunova, Veselina; Zhang, Yixin; Seidel, Ralf; Schwille, Petra
• Is Part Of: Angewandte Chemie. Volume 127:Number 22(2015)
• Page Start: 6601
• Page End: 6605
• Publication Date: 2015-04-16
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201501173
• Languages: English
• ISSNs: 0044-8249

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>We report a synthetic biology‐inspired approach for the engineering of amphipathic DNA origami structures as membrane‐scaffolding tools. The structures have a flat membrane‐binding interface decorated with cholesterol‐derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free‐standing lipid membranes, mimicking the biological activity of coat‐forming proteins, for example, from the I‐/F‐BAR family.</p> </abstract>