Improved Revascularization of Islet Grafts Using an Angiogenic Monocyte Subpopulation Derived From Spheroid Culture of Bone Marrow Mononuclear Cells. Issue 6 (9th April 2015)

• Record Type: Journal Article
• Authors: Oh, B. J.; Jin, S.‐M.; Choi, J.‐M.; Oh, S.‐H.; Shim, W.; Lee, M.‐S.; Lee, M.‐K.; Kim, J. H.
• Is Part Of: American journal of transplantation. Volume 15:Issue 6(2015:Jun.)
• Page Start: 1543
• Page End: 1554
• Publication Date: 2015-04-09
• Subjects: Transplantation of organs, tissues, etc -- Periodicals; 617.95
• DOI: 10.1111/ajt.13157
• Languages: English
• ISSNs: 1600-6135

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="ajt13157-sec-0001" sec-type="section"> <p>The spheroid culture method is an effective strategy for <italic>ex vivo</italic> expansion of an autologous therapeutic cell population. We investigated if cotransplantation of bone marrow–derived spheroids (BM‐spheroid) formed using 3D culture of BM‐derived mononuclear cells (BM‐MNCs) could improve the posttransplant outcome of islet grafts using a mouse syngeneic marginal mass renal subcapsular islet transplantation model. Using <italic>green fluorescent protein</italic> transgenic (GFP‐Tg) mice, the role of the BM‐spheroids and the contribution of vessels derived from donors and recipients in grafted areas were assessed by immunohistochemistry. Compared to fresh BM‐MNCs and nonspheroid remnant cells (BM‐nonspheroid), the BM‐spheroids, mainly composed of CXCR4<sup>+</sup>CD14<sup>+</sup> myeloid cells, showed higher angiogenic capacity, such as <italic>in vitro</italic> self‐formed vessel structures; increased expression of angiogenic and chemoattractive factors; and incorporation into new vessel formation in basement membrane matrix plugs. BM‐spheroid cotransplantation with islets improved the posttransplant outcomes in terms of glucose tolerance, serum insulin level, and diabetes reversal rate when compared with cotransplantation of BM‐nonspheroids. Immunohistochemistry revealed that cotransplantation of the BM‐spheroids increased vessel density, area of grafted endocrine and non‐endocrine tissue, and β cell proliferation. In conclusion, cotransplantation of islets and BM‐spheroids improved islet function through facilitation of revascularization and an increase in cell proliferation and islet cell mass.</p> </sec> </abstract>