Overexpression of a Novel Tumor Metastasis Suppressor Gene TMSG1/LASS2 Induces Apoptosis via a Caspase‐dependent Mitochondrial Pathway. Issue 7 (July 2015)

• Record Type: Journal Article
• Authors: Su, Jing; Yu, Wenjuan; Gong, Miaozi; You, Jiangfeng; Liu, Jianying; Zheng, Jie
• Is Part Of: Journal of cellular biochemistry. Volume 116:Issue 7(2015:Jul.)
• Page Start: 1310
• Page End: 1317
• Publication Date: 2015-07
• Subjects: Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.25086
• Languages: English
• ISSNs: 0730-2312

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jcb25086-sec-0001" sec-type="section"> <p>The tumor metastasis suppressor gene 1 (TMSG1), also designated homo sapiens longevity assurance homologue 2 of yeast LAG1 (LASS2), is a novel tumor metastatic suppressor gene. Although its effects on metastasis have been reported, its biological functions remain unclear. The purpose of this study was to investigate the effects of TMSG1/LASS2 protein on apoptosis and proliferation in human embryonic kidney cell lines HEK293 and 293 T and explore the potential mechanisms. Cell growth, morphology, expressions of apoptotic‐related proteins and cell cycle distribution were evaluated in HEK293 and 293 T cells transfected with TMSG1/LASS2 expression plasmids or vector controls. MTT assays showed that overexpression of TMSG1/LASS2 inhibited cell proliferation; and morphological observations and flow cytometric assays with Annexin V/propidium iodide showed TMSG1/LASS2 overexpression increased apoptosis in these cells. Western blot analysis demonstrated that overexpression of TMSG1/LASS2 resulted in the downregulation of Bcl‐2, release of cytochrome c from mitochondria, activation of procaspase‐9 and procaspase‐3, and the cleavage of PARP. Subsequent cell cycle analysis showed that TMSG1/LASS2 overexpression inhibited cell proliferation by mediating the induction of G0/G1 cell cycle arrest. Together, these results confirmed that <italic>TMSG1/LASS2</italic> is a potential metastasis suppressor gene, and suggested that the mechanism involved the induction of apoptosis and inhibition of cell proliferation via a caspase‐dependent mitochondrial pathway. J. Cell. Biochem. 116: 1310–1317, 2015. © 2015 Wiley Periodicals, Inc.</p> </sec> </abstract>