A New Enzyme‐linked Sorbent Assay (ELSA) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids. (4th March 2015)

• Record Type: Journal Article
• Authors: Göhner, Claudia; Weber, Maja; Tannetta, Dionne S.; Groten, Tanja; Plösch, Torsten; Faas, Marijke M.; Scherjon, Sicco A.; Schleußner, Ekkehard; Markert, Udo R.; Fitzgerald, Justine S.
• Is Part Of: American journal of reproductive immunology. Volume 73:Number 6(2015:Jun.)
• Page Start: 582
• Page End: 588
• Publication Date: 2015-03-04
• Subjects: Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12367
• Languages: English
• ISSNs: 1046-7408

Abstract

<abstract abstract-type="main" id="aji12367-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="aji12367-sec-0001" sec-type="section"> <title>Problem</title> <p>The pregnancy‐associated disease preeclampsia is related to the release of syncytiotrophoblast extracellular vesicles (STBEV) by the placenta. To improve functional research on STBEV, reliable and specific methods are needed to quantify them. However, only a few quantification methods are available and accepted, though imperfect. For this purpose, we aimed to provide an enzyme‐linked sorbent assay (ELSA) to quantify STBEV in fluid samples based on their microvesicle characteristics and placental origin.</p> </sec> <sec id="aji12367-sec-0002" sec-type="section"> <title>Method of Study</title> <p> <italic>Ex vivo</italic> placenta perfusion provided standards and samples for the STBEV quantification. STBEV were captured by binding of extracellular phosphatidylserine to immobilized annexin V. The membranous human placental alkaline phosphatase on the STBEV surface catalyzed a colorimetric detection reaction.</p> </sec> <sec id="aji12367-sec-0003" sec-type="section"> <title>Results and Conclusion</title> <p>The described ELSA is a rapid and simple method to quantify STBEV in diverse liquid samples, such as blood or perfusion suspension. The reliability of the ELSA was proven by comparison with nanoparticle tracking analysis.</p> </sec> </abstract>