The chromatin‐remodeling factor AtINO80 plays crucial roles in genome stability maintenance and in plant development. (May 2015)

• Record Type: Journal Article
• Authors: Zhang, Chi; Cao, Lin; Rong, Liang; An, Zengxuan; Zhou, Wangbin; Ma, Jinbiao; Shen, Wen‐Hui; Zhu, Yan; Dong, Aiwu
• Is Part Of: Plant journal. Volume 82:Number 4(2015:May)
• Page Start: 655
• Page End: 668
• Publication Date: 2015-05
• Subjects: Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.12840
• Languages: English
• ISSNs: 0960-7412

Abstract

<abstract abstract-type="main" id="tpj12840-abs-0001"> <title>Summary</title> <p>INO80 is a conserved chromatin‐remodeling factor in eukaryotes. While a previous study reported that the <italic>Arabidopsis thaliana </italic>INO80 (AtINO80) is required for somatic homologous recombination (HR), the role of <italic>AtINO80</italic> in plant growth and development remains obscure. Here, we identified and characterized two independent <italic>atino80</italic> mutant alleles, <italic>atino80‐5</italic> and <italic>atino80‐6, </italic> which display similar and pleiotropic phenotypes, including smaller plant and organ size, and late flowering. Under standard growth conditions, <italic>atino80‐5</italic> showed decreased HR; however, after genotoxic treatment, HR in the mutant increased, accompanied by more DNA double‐strand breaks and stronger cellular responses. Transcription analysis showed that many developmental and environmental responsive genes are overrepresented in the perturbed genes in <italic>atino80‐5</italic>. These genes significantly overlapped with the category of H2A.Z body‐enriched genes. AtINO80 also interacts with H2A.Z, and facilitates the enrichment of H2A.Z at the ends of the key flowering repressor genes <italic>FLC</italic> and <italic>MAF4/5</italic>. Our characterization of the <italic>atino80‐5</italic> and <italic>atino80‐6</italic> mutants confirms and extends the previous <italic>AtINO80</italic> study, and provides perspectives for linking studies of epigenetic mechanisms involved in plant chromatin stability with plant response to developmental and environmental cues.</p> </abstract>