Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice. (8th September 2014)

• Record Type: Journal Article
• Authors: Zhang, F.; Zhuo, D.‐L.; Zhang, F.; Huang, L.‐Y.; Wang, W.‐S.; Xu, J.‐L.; Vera Cruz, C.; Li, Z.‐K.; Zhou, Y.‐L.
• Is Part Of: Plant pathology. Volume 64:Number 3(2015:Jun.)
• Page Start: 568
• Page End: 575
• Publication Date: 2014-09-08
• Subjects: Agricultural pests -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1111/ppa.12283
• Languages: English
• ISSNs: 0032-0862

Abstract

<abstract abstract-type="main" id="ppa12283-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Bacterial blight (BB), caused by <italic>Xanthomonas oryzae</italic> pv. <italic>oryzae</italic> (Xoo), is one of the most devastating rice diseases worldwide. In this study, a rice introgression line (IL), FF329, which was identified from a BC<sub>1</sub>F<sub>4</sub> population derived from the cross between donor PSBRC66 (P66) and recipient Huang‐Hua‐Zhan (HHZ), exhibited a typical hypersensitive response (HR) when inoculated with all 21 representative Xoo strains, consisting of 14 Philippines races and seven Chinese pathotypes. By contrast, the parents were highly susceptible to 10 of the tested strains and resistant or moderately susceptible to 11 strains, but without HR symptoms. Genetic analysis of the F<sub>2</sub> population derived from the HHZ/FF329 cross with virulent Philippines race 6 (P6) and Chinese pathotype V (CV) revealed that BB resistance in FF329 was controlled by a single dominant gene. Because FF329 showed broad‐spectrum BB resistance, different from both parents, the gene identified was novel and was designated <italic>Xa39</italic>. Two SSR markers, RM21 and RM206, located on rice chromosome 11, were linked to the target gene by bulked segregant analysis. A further six markers linked to <italic>Xa39</italic> were identified in the region between RM21 and RM206 for fine gene mapping, and the <italic>Xa39</italic> locus was refined to a 97·4 kb interval flanked by markers RM26985 and DM13 using a large F<sub>2</sub> population. This gene provides rice breeders with a new option to incorporate BB resistance into newly developed cultivars.</p> </abstract>