Comparative genomics identifies the Magnaporthe oryzae avirulence effector AvrPi9 that triggers Pi9‐mediated blast resistance in rice. Issue 4 (6th February 2015)

• Record Type: Journal Article
• Authors: Wu, Jun; Kou, Yanjun; Bao, Jiandong; Li, Ya; Tang, Mingzhi; Zhu, Xiaoli; Ponaya, Ariane; Xiao, Gui; Li, Jinbin; Li, Chenyun; Song, Min‐Young; Cumagun, Christian Joseph R.; Deng, Qiyun; Lu, Guodong; Jeon, Jong‐Seong; Naqvi, Naweed I.; Zhou, Bo
• Is Part Of: New phytologist. Volume 206:Issue 4(2015)
• Page Start: 1463
• Page End: 1475
• Publication Date: 2015-02-06
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.13310
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph13310-abs-0001"> <title>Summary</title> <p> <list id="nph13310-list-0001" list-type="bullet"> <list-item> <p>We identified the <italic>Magnaporthe oryzae</italic> avirulence effector <italic>AvrPi9</italic> cognate to rice blast resistance gene <italic>Pi9</italic> by comparative genomics of requisite strains derived from a sequential planting method.</p> </list-item> <list-item> <p> <italic>AvrPi9</italic> encodes a small secreted protein that appears to localize in the biotrophic interfacial complex and is translocated to the host cell during rice infection. <italic>AvrPi9</italic> forms a tandem gene array with its paralogue proximal to centromeric region of chromosome 7. <italic>AvrPi9</italic> is expressed highly at early stages during initiation of blast disease.</p> </list-item> <list-item> <p>Virulent isolate strains contain Mg‐SINE within the <italic>AvrPi9</italic> coding sequence. Loss of <italic>AvrPi9</italic> did not lead to any discernible defects during growth or pathogenesis in <italic>M. oryzae</italic>. This study reiterates the role of diverse transposable elements as off‐switch agents in acquisition of gain‐of‐virulence in the rice blast fungus.</p> </list-item> <list-item> <p>The prevalence of <italic>AvrPi9</italic> correlates well with the avirulence pathotype in diverse blast isolates from the Philippines and China, thus supporting the broad‐spectrum resistance conferred by <italic>Pi9</italic> in different rice growing areas. Our results revealed that <italic>Pi9</italic> and <italic>Piz‐t</italic> at the <italic>Pi2/9</italic> locus activate race specific resistance by recognizing sequence‐unrelated <italic>AvrPi9</italic> and <italic>AvrPiz‐t</italic> genes, respectively.</p> </list-item> </list> </p> </abstract>