Novel In Vivo model for combinatorial fluorescence labeling in mouse prostate. Issue 9 (8th March 2015)

• Record Type: Journal Article
• Authors: Fang, Xiaolan; Gyabaah, Kenneth; Nickkholgh, Bita; Cline, J. Mark; Balaji, K.C.
• Is Part Of: Prostate. Volume 75:Issue 9(2015)
• Page Start: 988
• Page End: 1000
• Publication Date: 2015-03-08
• Subjects: Prostate -- Diseases -- Periodicals; 616
• DOI: 10.1002/pros.22984
• Languages: English
• ISSNs: 0270-4137

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="pros22984-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p>The epithelial layer of prostate glands contains several types of cells, including luminal and basal cells. Yet there is paucity of animal models to study the cellular origin of normal or neoplastic development in the prostate to facilitate the treatment of heterogenous prostate diseases by targeting individual cell lineages.</p> </sec> <sec id="pros22984-sec-0002" sec-type="section"> <title>METHODS</title> <p>We developed a mouse model that expresses different types of fluorescent proteins (XFPs) specifically in prostatic cells. Using an in vivo stochastic fluorescent protein combinatorial strategy, XFP signals were expressed specifically in prostate of Protein Kinase D1 (PKD1) knock‐out, K‐Ras<sup>G</sup><sup>12</sup><sup>D</sup> knock‐in, and Phosphatase and tensin homolog (PTEN) and PKD1 double knock‐out mice under the control of PB‐Cre promoter.</p> </sec> <sec id="pros22984-sec-0003" sec-type="section"> <title>RESULTS</title> <p>In vivo XFP signals were observed in prostate of PKD1 knock‐out, K‐Ras<sup>G</sup><sup>12</sup><sup>D</sup> knock‐in, and PTEN PKD1 double knock‐out mice, which developed normal, hyperplastic, and neoplastic prostate, respectively. The patchy expression pattern of XFPs in neoplasia tissue indicated the clonal origin of cancer cells in the prostate.</p> </sec> <sec id="pros22984-sec-0004" sec-type="section"> <title>CONCLUSIONS</title> <p>The transgenic mouse models demonstrate combinatorial fluorescent protein expression in normal and cancerous prostatic tissues. This novel prostate‐specific fluorescent labeled mouse model, which we named Prorainbow, could be useful in studying benign and malignant pathology of prostate. <italic>Prostate 75:988–1000, 2015</italic>. © 2015 Wiley Periodicals, Inc.</p> </sec> </abstract>