Regulatory role of collagen V in establishing mechanical properties of tendons and ligaments is tissue dependent. Issue 6 (27th April 2015)

• Record Type: Journal Article
• Authors: Connizzo, Brianne K.; Freedman, Benjamin R.; Fried, Joanna H.; Sun, Mei; Birk, David E.; Soslowsky, Louis J.
• Is Part Of: Journal of orthopaedic research. Volume 33:Issue 6(2015:Jun.)
• Page Start: 882
• Page End: 888
• Publication Date: 2015-04-27
• Subjects: Orthopedics -- Periodicals; Musculoskeletal system -- Periodicals; 616.7
• DOI: 10.1002/jor.22893
• Languages: English
• ISSNs: 0736-0266

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jor22893-sec-0001" sec-type="section"> <p>Patients with classic (type I) Ehlers–Danlos syndrome (EDS), characterized by heterozygous mutations in the <italic>Col5a1</italic> and <italic>Col5a2</italic> genes, exhibit connective tissue hyperelasticity and recurrent joint dislocations, indicating a potential regulatory role for collagen V in joint stabilizing soft tissues. This study asked whether the contribution of collagen V to the establishment of mechanical properties is tissue dependent. We mechanically tested four different tissues from wild type and targeted collagen V‐null mice: the flexor digitorum longus (FDL) tendon, Achilles tendon (ACH), the anterior cruciate ligament (ACL), and the supraspinatus tendon (SST). Area was significantly reduced in the <italic>Col5a1<sup>ΔTen/ΔTen</sup></italic> group in the FDL, ACH, and SST. Maximum load and stiffness were reduced in the <italic>Col5a1<sup>ΔTen/ΔTen</sup></italic> group for all tissues. However, insertion site and midsubstance modulus were reduced only for the ACL and SST. This study provides evidence that the regulatory role of collagen V in extracellular matrix assembly is tissue dependent and that joint instability in classic EDS may be caused in part by insufficient mechanical properties of the tendons and ligaments surrounding each joint. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:882–888, 2015.</p> </sec> </abstract>