Effect of crude extracts of selected actinomycetes on biofilm formation of A. schindleri, M. aci, and B. cereus. (19th August 2014)

• Record Type: Journal Article
• Authors: Saleem, Hafiz Ghulam Murtaza; Aftab, Usman; Sajid, Imran; Abbas, Zaigham; Sabri, Anjum Nasim
• Is Part Of: Journal of basic microbiology. Volume 55:issue 5(2015:May)
• Page Start: 645
• Page End: 651
• Publication Date: 2014-08-19
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201400358
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jobm201400358-sec-0001" sec-type="section"> <p>Actinomycetes are well known group of gram positive bacteria for their potential to produce antibiotics. This study sought to assess the ability of the selected actinomycetes to control biofilm forming bacteria isolated from different dental plaque samples. On the basis of morphological differences three out of ten different dental plaque bacterial isolates were selected for further study. These isolates were biochemically and genetically characterized and were identified as <italic>Acinetobacter schinndleri</italic>, <italic>Moraxella aci</italic>, and <italic>Bacillus cereus</italic>. Antibiotic resistant profile was measured through disc diffusion method and found that all three isolates were moderately sensitive to ofloxacin and erythromycin and resistant to trimethoprim. Antibacterial activity of ten different <italic>Streptomyces</italic> strains was assessed through an agar plug and well diffusion method against three dental biofilm forming bacteria. Two <italic>Streptomyces</italic> strains named as <italic>S. erythrogriseus</italic> and <italic>S. labedae</italic> showed good antibacterial activity against <italic>Moraxella</italic> and <italic>Acinetobacter</italic> strains. Ability of the four active antibiotic producing strains to inhibit biofilm formation was assessed using microtiter biofilm detection assay. It was found that biofilm forming ability of <italic>Acinetobacter</italic> and <italic>Moraxella</italic> was inhibited by <italic>S. labedae</italic> an antibiotic producing strain, while <italic>S. macrosporeus</italic> can only inhibit biofilm formation by <italic>B. cereus</italic>.</p> </sec> </abstract>