Mycoplasma hyorhinis induces proinflammatory responses in mice lymphocytes. (11th July 2014)

• Record Type: Journal Article
• Authors: Kornspan, Jonathan D.; Tsur, Maayan; Tarshis, Mark; Rottem, Shlomo; Brenner, Talma
• Is Part Of: Journal of basic microbiology. Volume 55:issue 5(2015:May)
• Page Start: 679
• Page End: 684
• Publication Date: 2014-07-11
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201400287
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jobm201400287-sec-0001" sec-type="section"> <p>Mycoplasmas are frequent contaminants of cultured cells, leading to alterations in cellular gene expression, protein synthesis, signal transduction, and metabolic pathways. <italic>Mycoplasma hyorhinis</italic>, the major contaminant of tissue cultures, has been implicated in a variety of diseases in swine. Most human and animal mycoplasmas remain attached to the surface of epithelial cells. Nonetheless, we have recently shown that <italic>M. hyorhinis</italic> is able to invade nonphagocytic melanoma cells. In the present study, we show by confocal laser scanning microscopy, that by exposing mice splenocytes to intact <italic>M. hyorhinis</italic>, intracellular mycoplasmas were detected. Mycoplasmal components were not detected within splenocytes after exposure to heat inactivated <italic>M. hyorhinis</italic> or to a purified <italic>M. hyorhinis</italic> lipoprotein (LPP) fraction. However, incubation of the splenocytes with intact <italic>M. hyorhinis</italic> cells, heat inactivated cells or <italic>M. hyorhinis</italic> LPP fraction induced accelerated cell proliferation and the secretion of interferon gamma and interleukin 17. Thus, <italic>M. hyorhinis</italic> and its LPPs can be added to the list of infectious agents causing direct stimulation of proinflammatory responses by mammalian lymphocytes.</p> </sec> </abstract>