Effect of different exercise protocols on metabolic profiles and fatty acid metabolism in skeletal muscle in high‐fat diet‐fed rats. (10th April 2015)

• Record Type: Journal Article
• Authors: Shen, Youqing; Xu, Xiangfeng; Yue, Kai; Xu, Guodong
• Is Part Of: Obesity. Volume 23:Number 5(2015:May)
• Page Start: 1000
• Page End: 1006
• Publication Date: 2015-04-10
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.21056
• Languages: English
• ISSNs: 1930-7381

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="oby21056-sec-0001" sec-type="section"> <title>Objective</title> <p>To evaluate the efficacy of mild‐intensity endurance, high‐intensity interval, and concurrent exercise on preventing high‐fat diet‐induced obesity.</p> </sec> <sec id="oby21056-sec-0002" sec-type="section"> <title>Methods</title> <p>Male rats were divided into five groups, control diet/sedentary group, high‐fat diet/sedentary, high‐fat diet/endurance exercise, high‐fat diet/interval exercise (HI), and high‐fat diet/concurrent exercise. All exercise groups were made to exercise for 10 weeks, with matched running distances. Body weight, fat content, blood metabolites, quantitative insulin sensitivity check index (QUICKI), and adipocyte and liver lipid droplet size were assessed, and the expression of fatty acid metabolism‐related genes was quantified.</p> </sec> <sec id="oby21056-sec-0003" sec-type="section"> <title>Results</title> <p>All exercise protocols reduced body weight, adiposity, serum triglycerides, and fasting glucose and also improved QUICKI to some extent. However, only HI prevented obesity and its associated pathologies completely. The expression of stearoyl‐coenzyme A desaturase‐1 was elevated in all rats fed a high‐fat diet whereas carnitine palmitoyltransferase 1 (<italic>CPT1</italic>) expression was increased with exercise. <italic>Rev‐erbα</italic> expression was elevated only in the HI group, which also had the highest level of <italic>CPT1</italic> expression.</p> </sec> <sec id="oby21056-sec-0004" sec-type="section"> <title>Conclusions</title> <p>The HI‐induced increase in <italic>Rev‐erbα</italic> and <italic>CPT1</italic> expression was associated with the complete prevention of diet‐induced obesity. Moreover, the increased caloric expenditure achieved with this protocol was preferential over other exercise regimens, and might be used to improve lipid metabolism.</p> </sec> </abstract>