Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats. (11th December 2014)

• Record Type: Journal Article
• Authors: Suzuki, Takehito; Fukuoka, Hideo; Ushikoshi, Setsuo; Sato, Reiichiro; Morita, Hidetoshi; Takizawa, Tatsuya
• Is Part Of: Animal science journal. Volume 86:Number 5(2015:May)
• Page Start: 532
• Page End: 540
• Publication Date: 2014-12-11
• Subjects: Animal culture -- Periodicals; Livestock -- Periodicals; 636
• DOI: 10.1111/asj.12328
• Languages: English
• ISSNs: 1344-3941

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Hepatoprotective effects of <italic>Rhizopus oryzae/</italic> U‐1 aqueous extract (RU) were demonstrated in carbon tetrachloride (CCl<sub>4</sub>)‐induced liver‐injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100 mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl<sub>4</sub> (125 mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities that were increased by the administration of CCl<sub>4</sub>. (ii) RU pretreatment (100 mg/kg) increased 5‐bromo‐2′‐deoxyuridine incorporation at 48 h after CCl<sub>4</sub> treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl<sub>4</sub> administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor (HGF) and a sustained and significant increase in insulin‐like growth factor‐I (IGF‐I) gene expression in hepatocytes injured by CCl<sub>4</sub> treatment. From these results, we conclude that oral pre‐administration of RU was effective to suppress liver injury induced by the subsequent oral CCl<sub>4</sub> administration, and RU‐induced increase in IGF‐I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.</p> </abstract>