Description of bovine major histocompatibility complex class IIa haplotypes using parthenogenetic embryo‐derived cells. (19th March 2015)

• Record Type: Journal Article
• Authors: Sá, A. L. A.; Leal, V. S. G.; Schneider, M. P. C.; Ohashi, O. M.; Santos, E. J. M.; Riggs, P. K.; Kochan, K. J.; Gill, C. A.; Downey, E. D.; Womack, J. E.; Skow, L. C.; Miranda, M. S.; Sena, L.
• Is Part Of: Animal genetics. Volume 46:Number 3(2015:Jun.)
• Page Start: 325
• Page End: 328
• Publication Date: 2015-03-19
• Subjects: Animal genetics -- Periodicals; 572.8
• DOI: 10.1111/age.12285
• Languages: English
• ISSNs: 0268-9146

Abstract

<abstract abstract-type="main" id="age12285-abs-0001"> <title>Summary</title> <p>In this study, we report an approach to characterize individual BoLA haplotypes using cells from parthenogenetic bovine embryos derived from slaughterhouse ovaries. Eight of the 15 parthenogenetic embryos so obtained had not undergone meiotic recombination on the BoLA region and were suitable to describe BoLA haplotypes. Detailed analysis of the BoLA class IIa region identified seven different class IIa haplotypes, including six not previously described and two new alleles of BoLA<italic>‐DQA</italic> and one BoLA<italic>‐DQB</italic>. Our method provided reliable sources of homozygous DNA to describe BoLA haplotypes.</p> </abstract>