Mediterranean semi-arid plant Astragalus armatus as a source of bioactive galactomannan. Issue 1 (January 2015)

• Record Type: Journal Article
• Authors: Boual, Zakaria; Pierre, Guillaume; Delattre, Cédric; Benaoun, Fatima; Petit, Emmanuel; Gardarin, Christine; Michaud, Philippe; El Hadj, Mohamed Didi Ould
• Is Part Of: Bioactive carbohydrates and dietary fibre. Volume 5:Issue 1(2015)
• Page Start: 10
• Page End: 18
• Publication Date: 2015-01
• Subjects: Functional foods -- Periodicals; Fiber in human nutrition -- Periodicals; Carbohydrates -- Periodicals; Glycoproteins -- Periodicals; Functional Food -- Periodicals; Dietary Fiber -- Periodicals; Carbohydrates -- Periodicals; Carbohydrates; Fiber in human nutrition; Functional foods; Glycoproteins; Periodicals; 613.28
• DOI: 10.1016/j.bcdf.2014.11.002
• Languages: English
• ISSNs: 2212-6198

Abstract

<abstract abstract-type="author" id="ab0005"> <title id="sect0005">Abstract</title> <sec> <p id="sp0045">A water-soluble polysaccharide fraction (WSPF) was extracted and purified from <italic>Astragalus armatus</italic> Lam. (Fabaceae), a spontaneous plant found in North Algerian Sahara. The structural features of WSPF were evaluated by colorimetric assays, Fourier transform infrared (FTIR) spectroscopy, high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and gas chromatography coupled to mass spectrometry (GC/MS). WSPF was a galactomannan-rich fraction, with a β-(1, 4)-<sc>d</sc>-man<italic>p</italic> backbone with α-(1, 6)-<sc>d</sc>-gal<italic>p</italic> branches, and a Man:Gal ratio of 1.13. Besides, the anti-complement activity and the antioxidant potential (DPPH and hydroxyl radicals) of WSPF were evaluated. The results exhibited an anti-complementary activity, with an IC₅₀ value of 0.39 mg/mL of the fraction, better than for heparin (0.58 mg/mL). Moreover, WSPF possessed a notable antioxidant activity on DPPH radical with an IC₅₀ of 330 µg/mL.</p> </sec> </abstract>