Analysis of the Roles of Glucose Transporter 1 and Hexokinase 2 in the Metabolism of Glucose by Extrahepatic Bile Duct Cancer Cells. (March 2015)

• Record Type: Journal Article
• Authors: Yoon, Sun Och; Jeon, Tae Joo; Park, Joon Seong; Ryu, Yong Hoon; Lee, Jae-Hoon; Yoo, Jung Sik; Kim, Jae Keun; Yoon, Dong Sup; Oh, Eun Ji
• Is Part Of: Clinical nuclear medicine. Volume 40:Number 3(2015)
• Publication Date: 2015-03
• Subjects: Nuclear medicine -- Periodicals; Radioisotope scanning -- Periodicals; Nuclear Medicine -- Periodicals; 616.07575
• DOI: 10.1097/RLU.0000000000000640
• Languages: English
• ISSNs: 0363-9762

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <sec> <title>Purpose of the Report</title> <p>Extrahepatic bile duct (EHD) cancer varies in uptake of FDG. The aim of the present study was to determine the role of glucose transporter (GLUT) 1 and hexokinase (HK) 2 in the glucose metabolism of EHD cancer cells using immunohistochemistry and <sup>18</sup>F-FDG PET/CT.</p> </sec> <sec> <title>Methods</title> <p>Twenty-six patients with EHD cancer who underwent baseline PET/CT and surgery were studied. Biopsies were immunohistochemically analyzed using antibodies against GLUT1 and HK2, and the expression was scored from 0 to 4 according to the percentage of stained cells. SUV and tumor-to-liver ratio (T/L<sub>ratio</sub>) were obtained from <sup>18</sup>F-FDG PET/CT data. SUV and T/L<sub>ratio</sub> and GLUT1 and HK2 expression were compared with histological grades and tumor locations (proximal and distal EHD) to correlate glucose metabolism with the expression of GLUT1 and HK2.</p> </sec> <sec> <title>Results</title> <p>SUV, T/L<sub>ratio</sub>, and GLUT1 and HK2 expression did not differ as a function of histological grade and tumor location. GLUT1 and HK2 were expressed in 20 (76.9%) and 22 (84.6%) of 26 tumor biopsies, respectively. The GLUT1 score, SUV, and T/L<sub>ratio</sub> increased, and the GLUT1 score, but not the HK2 score, correlated significantly with SUV (ρ = 0.648) and T/L<sub>ratio</sub> (ρ = 0.703). There was no direct correlation between the expression of GLUT1 and that of HK2 (ρ = 0.2046, <italic>P</italic> = 0.3161).</p> </sec> <sec> <title>Conclusions</title> <p>Although GLUT1 and HK2 regulate intracellular accumulation of FDG in many cancers, only GLUT1 expression was correlated with FDG uptake by EHD cancers.</p> </sec> </abstract>