Fluorine-18-deoxyglucose positron emission tomography/computed tomography with Ki67 and GLUT-1 immunohistochemistry for evaluation of the radiosensitization effect of oleanolic acid on C6 rat gliomas. Issue 1 (January 2015)

• Record Type: Journal Article
• Authors: Zhang, Yu; Xu, Huiqin; Wang, Hui; Yu, Wenjing; Zhao, Xuefeng; Xue, Yangyang
• Is Part Of: Nuclear medicine communications. Volume 36:Issue 1(2015:Jan.)
• Publication Date: 2015-01
• Subjects: Nuclear medicine -- Periodicals; 616.07575
• DOI: 10.1097/MNM.0000000000000211
• Languages: English
• ISSNs: 0143-3636

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <sec> <title>Objective</title> <p>The aim of this study was to investigate the radiosensitization effect of oleanolic acid (OA) in an in-vivo C6 rat glioma model using fluorine-18-deoxyglucose PET/computed tomography (<sup>18</sup>F-FDG PET/CT) and Ki67 and glucose transporter-1 (GLUT-1) immunohistochemistry (IHC) and evaluate the utility of <sup>18</sup>F-FDG PET/CT in assessing early changes after radiotherapy.</p> </sec> <sec> <title>Materials and methods</title> <p>Tumor-bearing rats were divided into four groups: the control group (group A), the OA group (group B), the radiotherapy group (group C), and the OA combined with radiotherapy group (group D). <sup>18</sup>F-FDG PET/CT images were obtained to monitor the tumor/muscle (T/M) ratio of <sup>18</sup>F-FDG uptake before treatment, 1 day after treatment, and 7 days after treatment. Tumor volume changes were also assessed, and hematoxylin and eosin staining and Ki67 and GLUT-1 IHC staining were also carried out.</p> </sec> <sec> <title>Results</title> <p>Before treatment, there were no obvious differences between the T/M ratios (<italic>F</italic>=0.147, <italic>P</italic>=0.931) and tumor volumes (<italic>F</italic>=0.177, <italic>P</italic>=0.911) among the four groups. At day 1 after treatment, statistical differences were observed in the T/M ratios (<italic>F</italic>=2.891, <italic>P</italic>=0.05), with decreased values in groups C and D compared with group A (<italic>t</italic><sub>CA</sub>=2.354, <italic>t</italic><sub>DA</sub>=2.356, <italic>P</italic><0.05). At day 7 after treatment, the T/M values of group D were lower than those of the other three groups (<italic>t</italic><sub>DC</sub>=2.416, <italic>P</italic>=0.03; <italic>t</italic><sub>DA</sub>=8.133, <italic>t</italic><sub>DB</sub>=7.656, <italic>P</italic><0.01), and the tumor volumes of group D were significantly decreased compared with group C (<italic>t</italic>=2.351, <italic>P</italic>=0.02). There were significant differences in tumor cell densities and in the expression of Ki67 and GLUT-1 in the four groups (<italic>F</italic>=16.667, 22.082, and 39.555, <italic>P</italic><0.001). The T/M ratio showed a significant correlation with the expression of Ki67 and GLUT-1, and our study also found a significant relationship between the expression of Ki67 and the expression of GLUT-1.</p> </sec> <sec> <title>Conclusion</title> <p>OA has a radiosensitization effect on C6 rat glioma tumors <italic>in vivo</italic>, detected using <sup>18</sup>F-FDG PET/CT and Ki67 and GLUT-1 IHC staining. <sup>18</sup>F-FDG PET/CT is a potentially sensitive tool for the evaluation of early changes after radiotherapy.</p> </sec> </abstract>