A C-type lectin (LvCTL4) from Litopenaeus vannamei is a downstream molecule of the NF-κB signaling pathway and participates in antibacterial immune response. Issue 1 (March 2015)

• Record Type: Journal Article
• Authors: Li, Haoyang; Chen, Yonggui; Li, Ming; Wang, Sheng; Zuo, Hongliang; Xu, Xiaopeng; Weng, Shaoping; He, Jianguo; Li, Chaozheng
• Is Part Of: Fish & shellfish immunology. Volume 43:Issue 1(2015:Mar.)
• Page Start: 257
• Page End: 263
• Publication Date: 2015-03
• Subjects: Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2014.12.024
• Languages: English
• ISSNs: 1050-4648

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">C-type lectins (CTLs) play multiple roles in innate immune defense against invading pathogens in both vertebrates and invertebrates. In this study, a new C-type lectin gene from pacific white shrimp <italic>Litopenaeus vannamei</italic> (designated as <italic>LvCTL4</italic>) was cloned by rapid amplification of the cDNA ends (RACE) method. The full-length cDNA of <italic>LvCTL4</italic> was 563 bp with open reading frame (ORF) of 471 bp encoding a polypeptide of 156 amino acids, including a putative signal sequence and a single C-type lectin-like domain (CTLD). The CTLD of 137 amino acid residues contained a mutated 'EPA' (Glu<sup>121</sup>-Pro<sup>122</sup>-Ala<sup>123</sup>) motif in the calcium-binding site 2 and three conserved disulfide bonds involved in structure maintenance. Tissue expression analysis showed <italic>LvCTL4</italic> was ubiquitously distributed with high levels in gill, intestine, epithelium and hepatopancreas. The expression of <italic>LvCTL4</italic> in gill was up-regulated in response to <italic>Vibrio parahaemolyticus</italic> challenge. RNAi knock-down of the <italic>LvCTL4</italic> gene significantly increased mortality after <italic>V. parahaemolyticus</italic> infection. A 103 bp 5′ flanking promoter sequence was obtained using the genome walking method and it contained a conserved NF-κB binding motif. Dual-Luciferase assay showed both <italic>LvDorsal</italic> and <italic>LvRelish</italic> could up regulate the promoter activity of <italic>LvCTL4</italic>. This is the first report that a shrimp C-type lectin can be regulated by both <italic>LvDorsal</italic> and <italic>LvRelish</italic>. These findings provided novel insights into the regulation of shrimp CTLs expression.</p> </sec> </abstract>