Characterization of a gC1qR from the giant freshwater prawn, Macrobrachium rosenbergii. Issue 1 (March 2015)

• Record Type: Journal Article
• Authors: Ye, Ting; Huang, Xin; Wang, Xian-Wei; Shi, Yan-Ru; Hui, Kai-Min; Ren, Qian
• Is Part Of: Fish & shellfish immunology. Volume 43:Issue 1(2015:Mar.)
• Page Start: 200
• Page End: 208
• Publication Date: 2015-03
• Subjects: Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2014.12.030
• Languages: English
• ISSNs: 1050-4648

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">gC1qR, as a multicompartmental and a multifunctional protein, plays an important role in innate immunity. In this study, a gC1qR homolog (<italic>MrgC1qR</italic>) in the giant freshwater prawn, <italic>Macrobrachium rosenbergii</italic> was identified. <italic>MrgC1qR</italic>, a 258-amino-acid polypeptide, shares high identities with gC1qR from other species. <italic>MrgC1qR</italic> gene was expressed in different tissues and was highest expressed in the hepatopancreas. In addition, the <italic>MrgC1qR</italic> transcript was significantly enhanced after 6 h of white spot syndrome virus (WSSV) infection or post 2 h, 24 h of <italic>Vibrio anguillarum</italic> challenge compared to appropriate controls. Moreover, recombinant MrgC1qR (rMrgC1qR) had bacterial binding activity, the result also revealed that rMrgC1qR could bind pathogen-associated molecular patterns (PAMPs) such as LPS or PGN, suggesting that MrgC1qRmight function as a pathogen-recognition receptor (PRR). Furthermore, glutathione <italic>S</italic>-transferase (GST) pull-down assays showed that rMrgC1qR with GST-tag could bind to rMrFicolin1 or rMrFicolin2 with His-tag. Altogether, these results may demonstrate a role for <italic>MrgC1qR</italic> in innate immunity in the giant freshwater prawns.</p> </sec> </abstract>