Establishment of an antibody avidity test to differentiate vaccinated cattle from those naturally infected with Mycoplasma bovis. Issue 1 (January 2015)

• Record Type: Journal Article
• Authors: Han, Xiaoxiao; Khan, Farhan Anwar; Zhu, Xifang; Zhang, Rui; Mustafa, Riaz; Hu, Changmin; Chen, Yingyu; Chen, Huanchun; Guo, Aizhen
• Is Part Of: Veterinary journal. Volume 203:Issue 1(2015)
• Page Start: 79
• Page End: 84
• Publication Date: 2015-01
• Subjects: Veterinary medicine -- Periodicals; 636
• DOI: 10.1016/j.tvjl.2014.10.032
• Languages: English
• ISSNs: 1090-0233

Abstract

<abstract abstract-type="author-highlights" id="ab0010"> <title id="st0010">Highlights</title> <sec> <p id="sp0010"> <list id="ulist0010"> <list-item id="u0010"> <label></label> <p id="p0010">An iELISA was established for <italic>Mycoplasma bovis</italic> and calibrated with a commercial kit.</p> </list-item> <list-item id="u0015"> <label></label> <p id="p0015">An NaSCN competitive iELISA was further developed to analyse IgG avidity.</p> </list-item> <list-item id="u0020"> <label></label> <p id="p0020">The avidity index (AI) cut-off value of the competitive iELISA was defined as 70.4%.</p> </list-item> <list-item id="u0025"> <label></label> <p id="p0025">The IgG avidity test differentiated naturally infected from vaccinated animals.</p> </list-item> <list-item id="u0030"> <label></label> <p id="p0030">Test sensitivity and specificity were 96.0% and 95.8%, respectively.</p> </list-item> </list> </p> </sec> </abstract>