Transcriptional profiles reveal a stepwise developmental program of memory CD8+ T cell differentiation. Issue 7 (11th February 2015)

• Record Type: Journal Article
• Authors: Roychoudhuri, Rahul; Lefebvre, Francois; Honda, Mitsuo; Pan, Li; Ji, Yun; Klebanoff, Christopher A.; Nichols, Carmen N.; Fourati, Slim; Hegazy, Ahmed N.; Goulet, Jean-Philippe; Gattinoni, Luca; Nabel, Gary J.; Gilliet, Michel; Cameron, Mark; Restifo, Nicholas P.; Sékaly, Rafick P.; Flatz, Lukas
• Is Part Of: Vaccine. Volume 33:Issue 7(2015)
• Page Start: 914
• Page End: 923
• Publication Date: 2015-02-11
• Subjects: Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2014.10.007
• Languages: English
• ISSNs: 0264-410X

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0045"> <list id="lis0010"> <list-item id="lsti0005"> <label></label> <p id="par0005">We perform whole transcriptome analysis of distinct CD8<sup>+</sup> T-cell memory populations arising endogenously following immunization with clinical and pre-clinical vaccines regimens.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">We find that transcriptional proximity relationships between endogenous <italic>T</italic><sub>N</sub>, <italic>T</italic><sub>CM</sub>, <italic>T</italic><sub>EM</sub> and <italic>T</italic><sub>EFF</sub> place memory cells at an intermediate state of differentiation between <italic>T</italic><sub>N</sub> and <italic>T</italic><sub>EFF</sub>.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">We compare transcriptional differences between <italic>T</italic><sub>N</sub>, <italic>T</italic><sub>CM</sub>, <italic>T</italic><sub>EM</sub> and <italic>T</italic><sub>EFF</sub> cells with known changes when CD8<sup>+</sup> T cells undergo repeated antigen stimulation.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">We find that at a single time-point following vaccination, cells can be found with the transcriptional imprint of distinct exposures to antigen.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">Our analysis favors the progressive differentiation model whereby cumulative antigen stimulation drives differentiation specifically from <italic>T</italic><sub>N</sub> > <italic>T</italic><sub>CM</sub> > <italic>T</italic><sub>EM</sub> > <italic>T</italic><sub>EFF</sub>.</p> </list-item> </list> </p> </sec> </abstract>