Evidence-based RT-PCR methods for the detection of the 8 most common MLL aberrations in acute leukemias. Issue 2 (February 2015)

• Record Type: Journal Article
• Authors: Burmeister, Thomas; Meyer, Claus; Gröger, Daniela; Hofmann, Julia; Marschalek, Rolf
• Is Part Of: Leukemia research. Volume 39:Issue 2(2015:Feb.)
• Page Start: 242
• Page End: 247
• Publication Date: 2015-02
• Subjects: Leukemia -- Periodicals; Leukemia -- Periodicals; Leucémie -- Périodiques; Leukemia; Periodicals; Electronic journals; Electronic journals; 616.9941905
• DOI: 10.1016/j.leukres.2014.11.017
• Languages: English
• ISSNs: 0145-2126

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0005"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">This paper describes RT-PCRs for detecting the eight most prevalent <italic>MLL</italic> aberrations.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">RT-PCR method covers around 90% of all <italic>MLL</italic>-r cases.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">PCR primers were based on the genomic analysis of more than 1.500 <italic>MLL</italic>-r cases.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">RT-PCRs detect also rarer transcript variants, in contrast to many previous methods.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">RT-PCRs can be performed in a convenient multiplex format.</p> </list-item> </list> </p> </sec> </abstract>