Detecting and preventing reversion to toxicity for a formaldehyde-treated C. difficile toxin B mutant. Issue 1 (1st January 2015)

• Record Type: Journal Article
• Authors: Wang, Bei; Wang, Su; Rustandi, Richard R.; Wang, Feng; Mensch, Christopher D.; Hong, Laura; Kristopeit, Adam; Secore, Susan; Dornadula, Geethanjali; Kanavage, Anthony; Heinrichs, Jon H.; Mach, Henryk; Blue, Jeffrey T.; Thiriot, David S.
• Is Part Of: Vaccine. Volume 33:Issue 1(2015)
• Page Start: 252
• Page End: 259
• Publication Date: 2015-01-01
• Subjects: Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2014.06.032
• Languages: English
• ISSNs: 0264-410X

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0045"> <list id="lis0010"> <list-item id="lsti0005"> <label></label> <p id="par0005">A <italic>C. difficile</italic> toxin B mutant with five mutations (mTcdB) has residual toxicity.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">Formaldehyde can remove residual toxicity but reversion in liquid can occur.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">In-vivo mouse toxicity and biochemical assays are sensitive indicators of reversion.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">Lyophilization after formaldehyde removal prevents mTcdB reversion to toxicity.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">Including formaldehyde can progressively modify mTcdB in the lyophilized state.</p> </list-item> </list> </p> </sec> </abstract>