MicroRNA-29b regulates migration in oral squamous cell carcinoma and its clinical significance. Issue 2 (February 2015)

• Record Type: Journal Article
• Authors: Yang, Cheng-Ning; Deng, Yi-Ting; Tang, Jia-Yu; Cheng, Shih-Jung; Chen, Szu-Ta; Li, Yue-Ju; Wu, Tai-Sheng; Yang, Muh-Hwa; Lin, Been-Ren; Kuo, Mark Yen-Ping; Ko, Jenq-Yuh; Chang, Cheng-Chi
• Is Part Of: Oral oncology. Volume 51:Issue 2(2015:Feb.)
• Page Start: 170
• Page End: 177
• Publication Date: 2015-02
• Subjects: Mouth -- Cancer -- Periodicals; Mouth -- Tumors -- Periodicals; Mouth Diseases -- Periodicals; Mouth Neoplasms -- Periodicals; Bouche -- Cancer -- Périodiques; Bouche -- Tumeurs -- Périodiques; Tumeurs -- Périodiques; Electronic journals; 616.9943105
• DOI: 10.1016/j.oraloncology.2014.10.017
• Languages: English
• ISSNs: 1368-8375

Abstract

<abstract xml:lang="en" abstract-type="author" id="ab005"> <title id="st110">Summary</title> <sec> <title id="st115">Objectives</title> <p id="sp0005">MicroRNA (miRNA) machinery regulates cancer cell behavior, and has been implicated in patients' clinical status and prognosis. We found that microRNA-29b (miR-29b) increased significantly in advanced migratory cells. However, miR-29b controls the migration ability, and its regulatory mechanism in oral squamous cell carcinoma (OSCC) remains unknown.</p> </sec> <sec> <title id="st120">Materials and methods</title> <p id="sp0010">We triggered miR-29b expression in OSCC patients and cell lines by conducting real-time quantitative PCR. We determined the functions of miR-29b in the migration of OSCC cells by using gain- and loss-of-function approaches. We elevated the target genes of miR29b through software predictions and a luciferase report assay. We used an orthotopic OSCC animal model to investigate the effects of miR29b on OSCC cell metastasis in vivo.</p> </sec> <sec> <title id="st125">Results</title> <p id="sp0015">The clinical data revealed that miR-29b expression was correlated with lymph node metastasis and an advanced tumor stage in 98 OSCC patients. Furthermore, multivariate analysis revealed that miR-29b expression was significantly correlated with recurrence, and indicated poor survival. MiR-29b promoted OSCC cell migration and downregulated CX3CL1, a cell–cell adhesion regulator, which plays an essential role in miR-29b-regulated OSCC cell migration machinery. Furthermore, we found that CX3CL1 expression was correlated with lymph node metastasis and an early tumor stage in OSCC patients, and negatively correlated with miR-29b expression.</p> </sec> <sec> <title id="st130">Conclusion</title> <p id="sp0020">MiR-29b acts as an oncomir, promoting cell migration through CX3CL1 suppression, and could be a potential therapeutic target for preventing OSCC progression.</p> </sec> </abstract>