Characterization of the biological activities and physicochemical characteristics of recombinant bovine interferon-α14. Issue 1 (March 2015)

• Record Type: Journal Article
• Authors: Shao, Jianwei; Cao, Chong; Bao, Jun; Gao, Mingchun; Wang, Junwei
• Is Part Of: Molecular immunology. Volume 64:Issue 1(2015:Mar.)
• Page Start: 163
• Page End: 169
• Publication Date: 2015-03
• Subjects: Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2014.11.011
• Languages: English
• ISSNs: 0161-5890

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0005"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">We amplified a BoIFN-α gene from bovine liver genomic DNA and named it as BoIFN-α_14.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">A recombinant mature BoIFN-α₁₄ was expressed in the yeast <italic>Pichia pastoris</italic>.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">The biological activities and physicochemical characteristics of BoIFN-α₁₄ were determined.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">BoIFN-α₁₄ could inhibit the CPE of VSV, and could inhibit cell proliferation.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">BoIFN-α₁₄ has the typical characteristics of IFN-α.</p> </list-item> </list> </p> </sec> </abstract>