Engineered hypoallergenic variants of osmotin demonstrate hypoallergenicity with in vitro and in vivo methods. Issue 1 (March 2015)

• Record Type: Journal Article
• Authors: Sharma, Prerna; Gaur, Shailendra Nath; Goel, Nitin; Arora, Naveen
• Is Part Of: Molecular immunology. Volume 64:Issue 1(2015:Mar.)
• Page Start: 46
• Page End: 54
• Publication Date: 2015-03
• Subjects: Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2014.10.019
• Languages: English
• ISSNs: 0161-5890

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0065"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">Osmotin structure was determined by homology modeling.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">Conformational IgE binding regions of osmotin were predicted.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">Mutants of osmotin were developed by site directed mutagenesis.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">Mutant Mabc showed complete abrogation of IgE binding with patients' sera.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">Mabc also demonstrated reduced allergenic potential in Balb/c mice.</p> </list-item> </list> </p> </sec> </abstract>