Cultured autologous keratinocytes in the treatment of large and deep burns: A retrospective study over 15 years. Issue 1 (February 2015)

• Record Type: Journal Article
• Authors: Auxenfans, Celine; Menet, Veronique; Catherine, Zulma; Shipkov, Hristo; Lacroix, Pierre; Bertin-Maghit, Marc; Damour, Odile; Braye, Fabienne
• Is Part Of: Burns. Volume 41:Issue 1(2015)
• Page Start: 71
• Page End: 79
• Publication Date: 2015-02
• Subjects: Burns and scalds -- Periodicals; 617.11
• DOI: 10.1016/j.burns.2014.05.019
• Languages: English
• ISSNs: 0305-4179

Abstract

<abstract abstract-type="author" id="abs0005"> <title id="sect0005">Abstract</title> <sec> <title id="sect0010">Aim</title> <p id="spar0005">The aim was to review the use and indications of cultured autologous epidermis (CAE) in extensive burns and to evaluate the efficiency of our strategy of burn treatment.</p> </sec> <sec> <title id="sect0015">Materials and methods</title> <p id="spar0010">This retrospective study comprised 15 years (1997–2012). <italic>Inclusion criteria</italic>: all patients who received CAE. <italic>Exclusion criteria</italic>: patients who died before complete healing and patients who received exclusively cultured allogeneic keratinocytes. Evaluation criteria were clinical. Time and success of wound healing after CAE graft were evaluated.</p> </sec> <sec> <title id="sect0020">Results</title> <p id="spar0020">A total of 63 patients were included with severity Baux score of 107 (from 70 to 140) and mean percentage of TBSA of 71% (from 40% to 97%). The CAE were used as Cuono method, in STSG donor sites and deep 2nd degree burns and in combination with large-meshed STSG (1:6–1:12) in extensively burned patients. Cuono method was used in 6 patients. The final take was 16% (0–30) because of the great fragility of the obtained epidermis. Nine patients with deep 2nd degree burns (mean TBSA 81%, from 60 to 97%) were successfully treated with only CAE without skin grafting. Combined technique (STSG meshed at 1:6–1:12 covered with CAE) was used in 27 patients (mean TBSA 69%, from 49% to 96%) with 85% success rate. Finally, donor sites treated with CAE in 49 patients could be harvested several times thanks to rapid epithelialization (time of wound healing was 7 days (from 5 to 10 days)).</p> </sec> <sec> <title id="sect0025">Conclusion</title> <p id="spar0025">The CAE allow rapid healing of STSG donor sites and deep 2nd second degree burns in extensively burned patients.</p> </sec> </abstract>