Hydrogen production by Enterobacter cloacae isolated from sugar refinery sludge. (21st January 2015)

• Record Type: Journal Article
• Authors: Sun, Lili; Huang, Aiyou; Gu, Wenhui; Ma, Yingchao; Zhu, Daling; Wang, Guangce
• Is Part Of: International journal of hydrogen energy. Volume 40:Number 3(2015)
• Page Start: 1402
• Page End: 1407
• Publication Date: 2015-01-21
• Subjects: Hydrogen as fuel -- Periodicals; Hydrogène (Combustible) -- Périodiques; Hydrogen as fuel; Periodicals; 665.81
• DOI: 10.1016/j.ijhydene.2014.11.121
• Languages: English
• ISSNs: 0360-3199

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">Hydrogen is recognized as a promising fuel for the future because it is renewable, environmentally harmless and cost-effective. Systematic research on the process of hydrogen production is important for increasing hydrogen yield and identification of suitable strains of bacteria. We investigated the hydrogen production pathways of <italic>Enterobacter cloacae</italic>. The maximum hydrogen yield was ∼707 ml L⁻¹, the H₂ content of the gas-phase was in the range 30–35% and the overall rate of hydrogen production was ∼70.70 ml h⁻¹ L⁻¹. Ethyl alcohol and 2, 3-butanediol were the major fermentative products, indicating the hydrogen-producing fermentation pathway of <italic>E. cloacae</italic> was the 2, 3-butanediol pathway. Proteins and enzymes involved in the 2, 3-butanediol pathway were generally detected by proteomics analysis, providing further evidence for the presence of the 2, 3-butanediol pathway in <italic>E. cloacae</italic>. Both ¹³C labeling experiments and proteomics analysis indicated the Embden-Meyerhof-Parnas (EMP) pathway was the major pathway and the hexose monophosphate (HMP) pathway had a weaker role, or did not participate, in glucose catalysis in <italic>E. cloacae</italic>.</p> </sec> </abstract>