Activation of the anticancer drugs cyclophosphamide and ifosfamide by cytochrome P450 BM3 mutants. Issue 1 (5th January 2015)

• Record Type: Journal Article
• Authors: Vredenburg, Galvin; den Braver-Sewradj, Shalenie; van Vugt-Lussenburg, Barbara M.A.; Vermeulen, Nico P.E.; Commandeur, Jan N.M.; Vos, J. Chris
• Is Part Of: Toxicology letters. Volume 232:Issue 1(2015)
• Page Start: 182
• Page End: 192
• Publication Date: 2015-01-05
• Subjects: Toxicology -- Periodicals; 363.179
• DOI: 10.1016/j.toxlet.2014.11.005
• Languages: English
• ISSNs: 0378-4274

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0055"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">We identified CYP BM3 mutants showing very efficient activation of CPA and IFA.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">CYP BM3 mutants showed very low formation of neuro- and nephrotoxic byproducts.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">Cytotoxicity of CPA and IFA metabolism was confirmed in human U2OS cells.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">CYP BM3 was used as a novel extracellular bioactivation tool in cellular assays.</p> </list-item> </list> </p> </sec> </abstract>