The effects of ROS in prostatic stromal cells under hypoxic environment. (June 2015)

• Record Type: Journal Article
• Authors: Ren, Hailin; Li, Xiaona; Cheng, Guojun; Li, Ning; Hou, Zhi; Suo, Jiming; Wang, Jian; Za, Xi
• Is Part Of: Aging male. Volume 18:Number 2(2015:Jun.)
• Page Start: 84
• Page End: 88
• Publication Date: 2015-06
• Subjects: Aging -- Periodicals; Men -- Periodicals; Gerontology -- Periodicals; Men -- Health and hygiene -- Periodicals; Aging -- periodicals; Geriatric -- periodicals; Men -- periodicals; Aged -- periodicals; Aged, 80 and over -- periodicals; 612.67
• DOI: 10.3109/13685538.2015.1018159
• Languages: English
• ISSNs: 1368-5538

Abstract

<abstract> <title>Abstract</title> <p> <italic>Objective</italic>: The objective of this study is to explore the effects of reactive oxygen species (ROS) under hypoxic environment in prostatic stromal cells (PSC).</p> <p> <italic>Methods and materials</italic>: To detect the expression of ROS in PSC and the tissues of benign prostatic hyperplasia (BPH) by flow cytometry; under hypoxic conditions, to observe the changes of cells growth and ROS in PSC; quantitative PCR was used to detect hypoxia inducible factor-1α (HIF-1α), androgen receptors (AR), vascular endothelial growth factor (VEGF), and interleukin-8 (IL-8) in PSC; After edaravone intervening, to examine the changes of cells growth, ROS, HIF-1α, AR, VEGF, and IL-8 under hypoxic conditions.</p> <p> <italic>Results</italic>: The expression of ROS in tissues and cells which under hypoxic condition was significantly increased. 3% O₂ promoted the proliferation. The HIF-1α, AR, VEGF, and IL-8 were upregulated under 3% O₂. After edaravone intervening, ROS significantly decreased, HIF-1α and VEGF were downregulated, and cell proliferation declined.</p> <p> <italic>Conclusions</italic>: Hypoxia stimulates the generation of ROS, and the ROS may play a key role in BPH.</p> </abstract>