The in vitro immunomodulatory effect of extracellular products (ECPs) of Vagococcus fluvialis L21 on European sea bass (Dicentrarchus labrax) leucocytes. Issue 2 (February 2015)

• Record Type: Journal Article
• Authors: Román, L.; Acosta, F.; Padilla, D.; El Aamri, F.; Bravo, J.; Vega, B.; Rodriguez, E.; Vega, J.; Déniz, S.; Real, F.
• Is Part Of: Fish & shellfish immunology. Volume 42:Issue 2(2015:Feb.)
• Page Start: 517
• Page End: 521
• Publication Date: 2015-02
• Subjects: Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2014.11.037
• Languages: English
• ISSNs: 1050-4648

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">The immune associated genes, interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor-α (TNF-α), ciclo-oxigenase-2 (COX-2), and Mx gene were studied by real-time PCR in head-kidney leucocytes of sea bass after incubation with the extracellular products (ECPs) of the probiotic strain <italic>Vagococcus fluvialis</italic> L21 and polyinosinic:polycytidylic acid (POLY I:C), at different times (T1.5, T6, T12, T24, T48 and T72). In general, we can observe how pro-inflammatory cytokines IL-1β, TNF-α, IL-6 and COX-2 studied displayed a strong peak after stimulation with 1.5 h of ECPs of <italic>V. fluvialis</italic> L21, significant differences (<italic>P</italic> < 0.05) exist with other periods and with the POLY I: C at the same time. Similarly to the case of IL-10 also produced a statistically significant (<italic>P</italic> < 0.05) peak of expression on leukocytes that were stimulated with the ECPs of <italic>V. fluvialis</italic> L21. In the case of Mx gene expression, we note that in almost all sampling times there is an up-regulation of the Mx gene in leucocytes incubated with ECPs and POLY I:C compared to the control and Mx expression was higher in leucocytes that were stimulated with the ECPs of <italic>V. fluvialis</italic> for all times, except in T24. With these results we can consider that the ECPs of <italic>V. fluvialis</italic> L21 have a great power of stimulating the <italic>in vitro</italic> expression of immune-related genes and may even be useful as adjuvants for vaccine in aquaculture.</p> </sec> </abstract>