Non-specific interaction of carbon nanotubes with the resazurin assay reagent: Impact on in vitro assessment of nanoparticle cytotoxicity. Issue 1 (February 2015)

• Record Type: Journal Article
• Authors: Breznan, Dalibor; Das, Dharani; MacKinnon-Roy, Christine; Simard, Benoit; Kumarathasan, Premkumari; Vincent, Renaud
• Is Part Of: Toxicology in vitro. Volume 29:Issue 1(2015)
• Page Start: 142
• Page End: 147
• Publication Date: 2015-02
• Subjects: Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2014.09.009
• Languages: English
• ISSNs: 0887-2333

Abstract

<abstract xml:lang="en" abstract-type="author-highlights" id="ab005"> <title id="st090">Highlights</title> <sec> <p id="sp0005"> <list id="l0005"> <list-item id="o0005"> <label></label> <p id="p0135">CNTs interfere with fluorescence-based resazurin cell viability assay.</p> </list-item> <list-item id="o0010"> <label></label> <p id="p0140">Physical quench can be circumvented by clarification of supernatants by centrifugation.</p> </list-item> <list-item id="o0015"> <label></label> <p id="p0145">Polar, oxidized CNTs but not pristine CNTs chemically quench resorufin fluorescence.</p> </list-item> <list-item id="o0020"> <label></label> <p id="p0150">Chemical interference can be easily quantified for elimination of the bias.</p> </list-item> </list> </p> </sec> </abstract>