Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models. Issue 1 (February 2015)

• Record Type: Journal Article
• Authors: Bonnier, F.; Keating, M.E.; Wróbel, T.P.; Majzner, K.; Baranska, M.; Garcia-Munoz, A.; Blanco, A.; Byrne, H.J.
• Is Part Of: Toxicology in vitro. Volume 29:Issue 1(2015)
• Page Start: 124
• Page End: 131
• Publication Date: 2015-02
• Subjects: Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2014.09.014
• Languages: English
• ISSNs: 0887-2333

Abstract

<abstract xml:lang="en" abstract-type="author-highlights" id="ab005"> <title id="st080">Highlights</title> <sec> <p id="sp0005"> <list id="l0005"> <list-item id="o0005"> <label></label> <p id="p0130">Protocols for cytotoxicological assessment of 3D cell cultures must be adapted.</p> </list-item> <list-item id="o0010"> <label></label> <p id="p0135">Collagen matrices induce a dilution of the effective cytotoxicity assay concentration.</p> </list-item> <list-item id="o0015"> <label></label> <p id="p0140">Diffusion and bioavailability of test substances in 3D matrices must be considered.</p> </list-item> <list-item id="o0020"> <label></label> <p id="p0145">Viability of immortalized cervical cells in 2D and 3D cell cultures are comparable.</p> </list-item> <list-item id="o0025"> <label></label> <p id="p0150">Results apply to cytotoxicity assays and to exposure protocols (nanoparticles/drugs).</p> </list-item> </list> </p> </sec> </abstract>