In vivo tracking and immunological properties of pulsed porcine monocyte-derived dendritic cells. Issue 2 (February 2015)

• Record Type: Journal Article
• Authors: Crisci, Elisa; Fraile, Lorenzo; Novellas, Rosa; Espada, Yvonne; Cabezón, Raquel; Martínez, Jorge; Cordoba, Lorena; Bárcena, Juan; Benitez-Ribas, Daniel; Montoya, María
• Is Part Of: Molecular immunology. Volume 63:Issue 2(2015:Feb.)
• Page Start: 343
• Page End: 354
• Publication Date: 2015-02
• Subjects: Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2014.08.006
• Languages: English
• ISSNs: 0161-5890

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0065"> <list id="lis0010"> <list-item id="lsti0025"> <label></label> <p id="par0300">Human or pig dendritic cells pulsed with superparamagnetic iron oxide particles did not alter their phenotypic and functional properties <italic>in vitro</italic> in the experimental conditions tested in this work.</p> </list-item> <list-item id="lsti0030"> <label></label> <p id="par0305">After subcutaneous inoculation in pigs, porcine SPIO-MoDC migration to regional lymph nodes was detected by MRI and confirmed by Perls staining of draining lymph nodes.</p> </list-item> <list-item id="lsti0035"> <label></label> <p id="par0310">Only one dose of virus-like particles-pulsed MoDCs in pigs was able to induce specific local and systemic responses.</p> </list-item> <list-item id="lsti0040"> <label></label> <p id="par0315">This proof-of-principle study shows the potential of using pigs as a suitable animal model to test DC trafficking with the aim of improving cellular therapies.</p> </list-item> </list> </p> </sec> </abstract>