The Arabidopsis leucine‐rich repeat receptor‐like kinase MUSTACHES enforces stomatal bilateral symmetry in Arabidopsis. (March 2015)

• Record Type: Journal Article
• Authors: Keerthisinghe, Sandra; Nadeau, Jeannette A.; Lucas, Jessica R.; Nakagawa, Tsuyoshi; Sack, Fred D.
• Is Part Of: Plant journal. Volume 81:Number 5(2015:Mar.)
• Page Start: 684
• Page End: 694
• Publication Date: 2015-03
• Subjects: Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.12757
• Languages: English
• ISSNs: 0960-7412

Abstract

<abstract abstract-type="main" id="tpj12757-abs-0001"> <title>Summary</title> <p>Stomata display a mirror‐like symmetry that is adaptive for shoot/atmosphere gas exchange. This symmetry includes the facing guard cells around a lens‐shaped and bilaterally symmetric pore, as well as radially arranged microtubule arrays that primarily originate at the pore and then grow outwards. Mutations in <italic>MUSTACHES</italic> (<italic>MUS</italic>), which encodes a leucine‐rich repeat receptor‐like kinase, disrupt this symmetry, resulting in defects ranging from skewed pores and abnormally focused and depolarized radial microtubule arrays, to paired guard cells that face away from each other, or a severe loss of stomatal shape. Translational <italic>MUSproMUS:tripleGFP</italic> fusions are expressed in cell plates in most cells types in roots and shoots, and cytokinesis and cell plates are mostly normal in <italic>mus</italic> mutants. However, in guard mother cells, which divide and then form stomata, <italic>MUS</italic> expression is notably absent from new cell plates, and instead is peripherally located. These results are consistent with a role for MUS in enforcing wall building and cytoskeletal polarity at the centre of the developing stoma via signalling from the vicinity of the guard cell membrane.</p> </abstract>