Molecular characterization, phylogenetic analysis and expression profiling of myoglobin and cytoglobin genes in response to heat stress in channel catfish Ictalurus punctatus. Issue 2 (21st January 2015)

• Record Type: Journal Article
• Authors: Feng, J. B.; Liu, S. K.; Wang, R. J.; Zhang, J. R.; Wang, X. L.; Kaltenboeck, L.; Li, J. L.; Liu, Z. J.
• Is Part Of: Journal of fish biology. Volume 86:Issue 2(2015)
• Page Start: 592
• Page End: 604
• Publication Date: 2015-01-21
• Subjects: Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.12584
• Languages: English
• ISSNs: 0022-1112

Abstract

<abstract abstract-type="main" id="jfb12584-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="jfb12584-para-0001">To understand the function of myoglobin (Mb) and cytoglobin (Cygb) in channel catfish <italic>Ictalurus punctatus</italic> in response to heat stress, <italic>mb</italic> and <italic>cygb</italic> genes were identified and characterized in this study. These genes were widely expressed in all the tested tissues, but strong tissue preferences were observed, with the <italic>mb</italic> gene being expressed most highly in the heart, <italic>cygb1</italic> most highly expressed in the intestine and <italic>cygb2</italic> most highly expressed in the brain. After heat‐stress challenge, <italic>mb</italic> and <italic>cygb</italic> genes were up‐regulated in almost all tested tissues. In general, such up‐regulation was more dramatic in the tolerant group than in the intolerant group, suggesting that higher expression of <italic>mb</italic> and <italic>cygb</italic> genes contributed to greater tolerance of <italic>I. punctatus</italic> to heat stress.</p> </abstract>